Assoc.Prof. Parinya Noisa

Year	Title	Journal
2021	Cordycepin-loaded Nanoparticles from Cassava Starch Promote the Proliferation of Submandibular Gland Cells and Inhibit the Growth of Oral Squamous Carcinoma Cells Kaokaen P., Jaiboonma A., Chaicharoenaudomrung N., Kunhorm P., Janebodin K., Noisa P., Jitprasertwong P.	Nutrition and Cancer
Abstract: This study examined associations between the effect of treatment with nano-cassava starch that contained cordycepin (CS) extract, targeting human submandibular gland cells (HSGs), and human oral squamous carcinoma cells (HSC-4). Cassava starch nanoparticles (CSNPs) were prepared by either physical or acid treatment. These nanoparticles were then loaded with either CS or cordyceps medium and then treated with HSG or HSC-4 cells in different concentrations of CS and nanoparticles. Moreover, the protein secretion, reactive oxygen species (ROS) activity and the expression of salivary-specific genes, antioxidant gene were determined after treatment. CSNPs can enhance the activity of CS at low concentrations. Cordycepin-loaded cassava starch nanoparticles (CCSNPs) increased HSG proliferation, protein secretion, and the expression of salivary-specific genes, AMY and AQP5. Besides, CCSNPs also protected and scavenged of ROS via the stimulation of the antioxidant genes in HSGs, indicating the protective roles of CS to HSGs. On the other hand, CCSNPs inhibited the growth of HSC-4 cells by stimulating ROS generation and reducing protein secretion. This finding suggested that CCSNPs presented the dual actions against HSGs and human oral squamous carcinoma cells, and the encapsulation of CS with cassava nanoparticles enhanced the activity of CS. © 2020 Taylor & Francis Group, LLC. Keyword: Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091009982&doi=10.1080%2f01635581.2020.1819350&partnerID=40&md5=5420bf3442ddbfdb41361dbbe15f4f67 DOI: 10.1080/01635581.2020.1819350
2020	Near-Infrared Fluorescent pH Responsive Probe for Targeted Photodynamic Cancer Therapy Siriwibool S., Kaekratoke N., Chansaenpak K., Siwawannapong K., Panajapo P., Sagarik K., Noisa P., Lai R.-Y., Kamkaew A.	Scientific Reports
Abstract: We developed a pH dependent amino heptamethine cyanine based theranostic probe (I2-IR783-Mpip) that can be activated by near infrared light. I2-IR783-Mpip, in acidic condition, exhibited an intense, broad NIR absorption band (820–950 nm) with high singlet oxygen generation upon exposure to NIR light (~850 nm). Theoretical calculations showed that the protonation of the probe in an acidic environment decreased the molecular orbital energy gaps and increased the intramolecular charge transfer efficiency. I2-IR783-Mpip exhibited good photodynamic efficiency towards liver hepatocellular carcinoma cells under physiological and slightly acidic conditions while normal human embryonic kidney cells remained alive under the same conditions. Detection of intracellular reactive oxygen species (ROS) in cells treated with I2-IR783-Mpip after NIR light exposure confirmed PDT efficiency of the probe in acidic environment. Moreover, I2-IR783-Mpip also demonstrated efficient phototoxicity under deep-seated tumour cell system. We believed this is the first PDT agent that possesses intrinsic tumour binding and selectively eradicate tumour in acidic environment under 850 nm NIR lamp. © 2020, The Author(s). Keyword: Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078452141&doi=10.1038%2fs41598-020-58239-5&partnerID=40&md5=3d9dcb15a439465744f64db46f676bdd DOI: 10.1038/s41598-020-58239-5
2020	Cordycepin attenuates salivary hypofunction through the prevention of oxidative stress in human submandibular gland cells Jaiboonma A., Kaokaen P., Chaicharoenaudomrung N., Kunhorm P., Janebodin K., Noisa P., Jitprasertwong P.	International Journal of Medical Sciences
Abstract: Xerostomia (dry mouth) is a significant age-related condition. Meanwhile, cordycepin, the natural therapeutic agent, has demonstrated an anti-aging effect. Therefore, the present study aimed to investigate the preventive effects of cordycepin on secretory function in an in vitro model of hydrogen peroxide (H2O2)-induced salivary hypofunction. After being exposed to H2O2, human submandibular gland (HSG) cells were treated with various concentrations of cordycepin (6.25-50 µM) for 24, 48, and 72h. To evaluate cell proliferation and reactive oxygen species (ROS) generation, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide and 2, 7’-dichlorodihydrofluorescein diacetate assays were performed. The amylase activity was kinetically measured by 2-chloro-p-nitrophenol linked with maltotrioside. The expression of salivary, antioxidant and apoptotic markers at mRNA and protein levels were performed by reverse transcriptase polymerase chain reaction (RT-PCR) and immunofluorescence analysis, respectively. We demonstrated that cordycepin (6.25-25 µM) contributed to significant increases in expression of the salivary marker genes, alpha-amylase 1 (AMY1A) and aquaporin-5 (AQP5), and in amylase secretion without changes in cell viability. Under oxidative stress, HSG cells showed remarkable dysfunction. Cordycepin rescued the protective effects partially by decreasing ROS generation and restoring the expression of the salivary proteins, AMY and AQP5 via anti-oxidant and anti-apoptotic activity. In addition, the amount of amylase that was secreted from HSG cells cultured in cordycepin was increased. In conclusion, cordycepin demonstrated a protective effect on H2O2-induced HSG cells by decreasing ROS generation and upregulating the salivary function markers, AMY1A and AQP5, at both the transcriptional and translational levels. © The author(s). Keyword: Cordycepin; Dry mouth; Saliva; Salivary gland; Xerostomia Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087929033&doi=10.7150%2fijms.46707&partnerID=40&md5=a51b4c122810763d8bf61d9fd3e72911 DOI: 10.7150/ijms.46707
2020	Transcriptomic profiling of 3D glioblastoma tumoroids for the identification of mechanisms involved in anticancer drug resistance Chaicharoenaudomrung N., Kunhorm P., Promjantuek W., Rujanapun N., Heebkaew N., Soraksa N., Noisa P.	In Vivo
Abstract: Background/Aim: Among various types of brain tumors, glioblastoma is the most malignant and highly aggressive brain tumor that possesses a high resistance against anticancer drugs. To understand the underlined mechanisms of tumor drug resistance, a new and more effective research approach is required. The three dimensional (3D) in vitro cell culture models could be a potential approach to study cancer features and biology, as well as screen for anticancer agents due to the close mimicry of the 3D tumor microenvironments. Materials and Methods: With our developed 3D alginate scaffolds, Ilumina RNA-sequencing was used to transcriptomically analyze and compare the gene expression profiles between glioblastoma cells in traditional 2-dimensional (2D) monolayer and in 3D Ca-alginate scaffolds at day 14. To verify the reliability and accuracy of Illumina RNA-Sequencing data, ATP-binding cassette transporter genes were chosen for quantitative real-time polymerase chain reaction) verification. Results: The results showed that 7,411 and 3,915 genes of the 3D glioblastoma were up-regulated and down-regulated, respectively, compared with the 2D-cultured glioblastoma. Furthermore, the Kyoto Encyclopaedia of Genes and Genomes pathway analysis revealed that genes related to the cell cycle and DNA replication were enriched in the group of down-regulated gene. On the other hand, the genes involved in mitogen-activated protein kinase signaling, autophagy, drug metabolism through cytochrome P450, and ATP-binding cassette transporter were found in the up-regulated gene collection. Conclusion: 3D glioblastoma tumoroids might potentially serve as a powerful platform for exploring glioblastoma biology. They can also be valuable in anti-glioblastoma drug screening, as well as the identification of novel molecular targets in clinical treatment of human glioblastoma. © 2020 International Institute of Anticancer Research. All rights reserved. Keyword: 3D tumoroids; Anticancer drug screening; Glioblastoma; Transcriptomics Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077261037&doi=10.21873%2finvivo.11762&partnerID=40&md5=3f2e0b0680422fe76364795ee1d39d63 DOI: 10.21873/invivo.11762
2019	Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling Chaicharoenaudomrung N., Kunhorm P., Noisa P.	World Journal of Stem Cells
Abstract: Three-dimensional (3D) culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures. In cancer and stem cell research, the natural cell characteristics and architectures are closely mimicked by the 3D cell models. Thus, the 3D cell cultures are promising and suitable systems for various proposes, ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives. This review provides a comprehensive compendium of recent advancements in culturing cells, in particular cancer and stem cells, using 3D culture techniques. The major approaches highlighted here include cell spheroids, hydrogel embedding, bioreactors, scaffolds, and bioprinting. In addition, the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed, and the prominent studies of 3D cell culture systems were discussed. © The Author(s) 2019. Keyword: Cancer; Disease modeling; In vitro screening platform; Stem cells; Three-dimensional cultures Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076719467&doi=10.4252%2fwjsc.v11.i12.1065&partnerID=40&md5=b47aad2e71721058638023b5c05ec244 DOI: 10.4252/wjsc.v11.i12.1065
2019	Fabrication of 3D calcium-alginate scaffolds for human glioblastoma modeling and anticancer drug response evaluation Chaicharoenaudomrung N., Kunhorm P., Promjantuek W., Heebkaew N., Rujanapun N., Noisa P.	Journal of Cellular Physiology
Abstract: The three-dimensional (3D) cell culture model has been increasingly used to study cancer biology and screen for anticancer agents due to its close mimicry to in vivo tumor biopsies. In this study, 3D calcium(Ca)-alginate scaffolds were developed for human glioblastoma cell culture and an investigation of the responses to two anticancer agents, doxorubicin and cordycepin. Compared to the 2D monolayer culture, glioblastoma cells cultured on these 3D Ca-alginate scaffolds showed reduced cell proliferation, increased tumor spheroid formation, enhanced expression of cancer stem cell genes (CD133, SOX2, Nestin, and Musashi-1), and improved expression of differentiation potential-associated genes (GFAP and β-tubulin III). Additionally, the vascularization potential of the 3D glioblastoma cells was increased, as indicated by a higher expression of tumor angiogenesis biomarker (VEGF) than in the cells in 2D culture. To highlight the application of Ca-alginate scaffolds, the 3D glioblastomas were treated with anticancer agents, including doxorubicin and cordycepin. The results demonstrated that the 3D glioblastomas presented a greater resistance to the tested anticancer agents than that of the cells in 2D culture. In summary, the 3D Ca-alginate scaffolds for glioblastoma cells that were developed in this study offer a promising platform for anticancer agent screening and the discovery of drug-resistant mechanisms of cancer. © 2019 Wiley Periodicals, Inc. Keyword: 3D brain cancer; 3D calcium-alginate scaffolds; anticancer drug screening; brain cancer model; glioblastoma Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069740834&doi=10.1002%2fjcp.28608&partnerID=40&md5=5912c5b9c8690d7fd444e1e13c4e900a DOI: 10.1002/jcp.28608
2019	Small molecules re-establish neural cell fate of human fibroblasts via autophagy activation Rujanapun N., Heebkaew N., Promjantuek W., Sotthibundhu A., Kunhorm P., Chaicharoenaudomrung N., Noisa P.	In Vitro Cellular and Developmental Biology - Animal
Abstract: The generation of neural cells is of great interest in medical research because of its promising in neurodegenerative diseases. Small chemical molecules have been used for inducing specific cell types across lineage boundaries. Therefore, to direct neural cell fate, small molecule is a feasible approach for generating clinically relevant cell types without genetic alterations. Human fibroblasts have been directly induced into neural cells with different combinations of small molecules; however, the mechanism underlying neural induction is still not fully understood. In this study, human fibroblasts were induced into neural cells by using only 4 small molecules in a short time period, 5 d. Small molecules used in this study included WNT activator, DNMT inhibitor, Notch inhibitor, and retinoic acid. Neural-specific genes, including NESTIN, TUJ1, and SOX2, were upregulated upon the induction for 5 d. Noteworthy, this neural induction process by small molecules coincided with the activation of autophagy. Autophagy-related genes, such as LC3, ATG12, and LAMP1, were enhanced upon neural induction, and the number of induced-neural cells decreased when autophagy was suppressed by chloroquine. The activation of autophagy was found to reduce ROS generation within the induced-neural cells, and the inhibition of autophagy by chloroquine suppressed the expression of antioxidant genes, CATALASE, SOD, and GPX. This implied that autophagy maintained the optimal level of ROS for neural induction of human fibroblasts. Altogether, this study presented the effective and convenient condition to induce neural cells from human fibroblasts and revealed the positive roles of autophagy in controlling neural cell induction. © 2019, The Society for In Vitro Biology. Keyword: Autophagy; Human fibroblasts; Neural induction; Small molecules Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069478170&doi=10.1007%2fs11626-019-00381-0&partnerID=40&md5=ea3b4d49b4703c9ecf7fd6f05932ff70 DOI: 10.1007/s11626-019-00381-0
2019	Amine-Functionalized and Hydroxyl-Functionalized Magnesium Ferrite Nanoparticles for Congo Red Adsorption Aoopngan C., Nonkumwong J., Phumying S., Promjantuek W., Maensiri S., Noisa P., Pinitsoontorn S., Ananta S., Srisombat L.	ACS Applied Nano Materials
Abstract: In this paper, the potential use of either amine-functionalized or hydroxyl-functionalized magnesium ferrite (MgFe2O4) nanoparticles (NPs) as Congo red nanoadsorbents is explored and compared. The amine-functionalized MgFe2O4 NPs (denoted as MgFe2O4-NH2 NPs) were synthesized by a one-pot coprecipitation method using ethanolamine as a surface modifier, while the hydroxyl-functionalized MgFe2O4 NPs (denoted as MgFe2O4-OH NPs) were prepared by a hydrothermal method. In general, both nanoadsorbents can be successfully produced without calcination and were found to possess superparamagnetic properties with high saturation magnetization (Ms). In particular, MgFe2O4-OH NPs exhibit a higher Ms value of ∼53 emu g-1, promoting the rapid separation ability of the NPs from the treated solution using an external permanent magnet. The Congo red removal performance of these nanoadsorbents was investigated as a function of the pH of the aqueous solution and contact time. The removal efficiency of Congo red by MgFe2O4-NH2 NPs was found to be ∼96% within 180 min at pH 6, while MgFe2O4-OH NPs provided a removal efficiency at ∼88% within 420 min at pH 8. In addition, the maximum adsorption capacities (qm) calculated using the Langmuir isotherm equation were found to be 71.4 and 67.6 mg g-1 for MgFe2O4-NH2 and MgFe2O4-OH NPs, respectively. The higher qm value of MgFe2O4-NH2 NPs could be attributed to stronger electrostatic interactions with the sulfonate groups of Congo red formed by larger numbers of protonated amine groups than protonated hydroxyl groups of the adsorbents under the performed conditions. Moreover, reusability experiments also revealed that MgFe2O4-NH2 NPs offered a higher removal efficiency than MgFe2O4-OH NPs for the same cycles tested. Therefore, this study demonstrates that MgFe2O4-NH2 NPs synthesized by a simple one-pot synthetic method are applicable as reusable magnetic nanoadsorbents for Congo red removal in current practice. Copyright © 2019 American Chemical Society. Keyword: adsorption; amine-functionalized nanoparticles; Congo red; hydroxyl-functionalized nanoparticles; magnetic nanoadsorbents; MgFe2O4 nanoparticles Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078554647&doi=10.1021%2facsanm.9b01305&partnerID=40&md5=2d7f4649aaa5fdff1f2599d9765daea4 DOI: 10.1021/acsanm.9b01305
2019	Enrichment of cordycepin for cosmeceutical applications: culture systems and strategies Kunhorm P., Chaicharoenaudomrung N., Noisa P.	Applied Microbiology and Biotechnology
Abstract: Cordyceps spp. is the herbal medication initially used in China and has been reported as the unique resource of cordycepin. Cordycepin exhibits many health benefits, including anti-photoaging and anti-pigmentation; therefore, it potentially is a bioactive ingredient of cosmetic products. In order to enrich cordycepin content in Cordyceps, two artificial cultivation procedures, which are solid-state fermentation and liquid culture, were developed and optimized. The aim of this review is to illustrate cordycepin biosynthesis pathway in Cordyceps, and its bioactivity for cosmeceutical applications, as well as comparing the two different cultivation procedures. The basic model of artificial cultivation of Cordyceps is introduced; meanwhile, the potential application of modern biotechnology to the artificial cultivation is also discussed. This review should be of interest to the readers for the development of cordycepin bioproduction in order to be applied in cosmeceutical industry and some other uses. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Keyword: Cordycepin; Cordyceps; Cosmetics; Liquid culture; Solid-state fermentation Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060154654&doi=10.1007%2fs00253-019-09623-3&partnerID=40&md5=9b9853dd40234210bbc66a1ccd4d177f DOI: 10.1007/s00253-019-09623-3
2019	Aza-BODIPY probe for selective visualization of cyclooxygenase-2 in cancer cells Pewklang T., Chansaenpak K., Lai R.-Y., Noisa P., Kamkaew A.	RSC Advances
Abstract: AZB-IMC2 was developed as a COX-2 specific probe that exhibited a brighter fluorescence signal in cancer cells that overexpress COX-2 compared to normal cells. Oxidative stress agent-treated inflamed cell lines inducing high COX-2 levels revealed an enhanced fluorescence signal. Inhibitory studies showed a markedly reduced fluorescence intensity in cancer cells. The results suggested that AZB-IMC2 could be developed as a promising molecular tool for imaging guiding during surgery. © The Royal Society of Chemistry. Keyword: Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065494783&doi=10.1039%2fc9ra01948k&partnerID=40&md5=f0b0c08277d92a541077a4e9f3280e34 DOI: 10.1039/c9ra01948k