This study proposed a kinetic model for succinate production from xylose and glucose by Escherichia coli KJ12201 based on the Monod equation in which inhibitions of xylose or glucose and succinate were simultaneously considered. Our proposed model showed the strong inhibition effect by xylose on the specific growth rate while glucose had no inhibitory effect. Additionally, maximum concentrations of succinate that completely inhibited the cell growth were 60.70 g/L for xylose and 82.71 g/L for glucose. The kinetic parameters of the proposed model were determined by minimizing the gap between experimental data and the model prediction. With estimated kinetic parameters, values solved by the proposed model aligned and suited experimental data in the range of substrate concentrations (20–80 g/L) used. The sensitivity analysis also revealed that the maximum specific growth rate (µmax) and the maximum inhibitory xylose concentration (C*xylose) provided extreme influences on the succinate production. Our proposed model was then validated and found that it precisely predicted the cell growth, substrate utilization and succinate production by the strain. The proposed model may be employed for effectively controlling and optimizing the succinate production from other substrates containing glucose and xylose in a large-scale process by E. coli KJ12201. © 2022 Elsevier B.V.