The objectives of this study were to survey 1) student attitudes, 2) student learning behaviors, and 3) satisfaction of students toward using e-Courseware in a flipped classroom teaching and learning approach of the 312340 Application of Biotechnology in Crop Production Course at Suranaree University of Technology. A sample group was obtained by purposive sampling and consisted of 44 fourth year undergraduate students in the School of Crop Production Technology who registered for the course in the first trimester of the academic year 2018. From the results of the questionnaire on student attitudes toward using e-Courseware in a flipped classroom teaching and learning approach, compared to the traditional lecture-based teaching and learning approach, using a 5 level rating scale (1 = the lowest to 5 = the highest), it was found that 37 students (84.1%) replied with significantly higher scores for their attitudes towards using e-Courseware in a flipped classroom approach compared to the traditional lecture-based approach in all aspects (p < 0.01) except learning difficulty (p = 0.104), resulting in a higher overall attitude score (4.36 ± 0.72), compared to that of the traditional lecture-based approach (3.01 ± 0.88). Although the score for learning difficulty, rated for using e-Courseware in a flipped classroom approach tended to be higher (3.71 ±0.93) than the score of 3.29 ±0.92 for a traditional lecture-based approach, 94.6% students still expressed their preference for using e-Courseware in a flipped classroom approach for future courses. From the behavior observation form for students using e-Courseware in a flipped classroom teaching and learning approach, using a 3 level rating scale (1 = low to 3 = high), it was found that learners' participation in the learning activities was at a high level (2.59 ±0.50) on average and learning behavior was also at a high level (2.63 ± 0.49) on average. The questionnaire on student satisfaction on using e-Courseware in a flipped classroom teaching and learning approach, using a 5 level rating scale (1 = the lowest to 5 = the highest) revealed that 32 students (72.7%) replied with the following ratings: for overall satisfaction, the rating was at the highest level (4.38 ±0.66), and for the usefulness of e-Courseware for teaching and learning, the rating was also at the highest level (4.59 ± 0.67). These results indicate positive attitudes, learning behaviors and satisfaction of the students toward using e-Courseware in a flipped classroom teaching and learning approach, suggesting the effectiveness and usefulness of this approach for improvements in future courses. © 2019 Association for Computing Machinery.