Assoc.Prof. Rangsun Parnpai

Year	Title	Journal
2017	Induced Pluripotent HD Monkey Stem Cells Derived Neural Cells for Drug Discovery Kunkanjanawan T., Carter R., Ahn K.-S., Yang J., Parnpai R., Chan A.W.S.	SLAS Discovery
Abstract: Huntington’s disease (HD) is a neurodegenerative disease caused by an expansion of CAG trinucleotide repeat (polyglutamine [polyQ]) in the huntingtin (HTT) gene, which leads to the formation of mutant HTT (mHTT) protein aggregates. In the nervous system, an accumulation of mHTT protein results in glutamate-mediated excitotoxicity, proteosome instability, and apoptosis. Although HD pathogenesis has been extensively studied, effective treatment of HD has yet to be developed. Therapeutic discovery research in HD has been reported using yeast, cells derived from transgenic animal models and HD patients, and induced pluripotent stem cells from patients. A transgenic nonhuman primate model of HD (HD monkey) shows neuropathological, behavioral, and molecular changes similar to an HD patient. In addition, neural progenitor cells (NPCs) derived from HD monkeys can be maintained in culture and differentiated to neural cells with distinct HD cellular phenotypes including the formation of mHTT aggregates, intranuclear inclusions, and increased susceptibility to oxidative stress. Here, we evaluated the potential application of HD monkey NPCs and neural cells as an in vitro model for HD drug discovery research. © 2016, © 2016 Society for Laboratory Automation and Screening. Keyword: drug screening; Huntington’s disease; monkey model; neural cells; pluripotent stem cells Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021116818&doi=10.1177%2f2472555216685044&partnerID=40&md5=609b980f121839e2158884cd0036a131 DOI: 10.1177/2472555216685044
2017	Effect of medium additives during liquid storage on developmental competence of in vitro matured bovine oocytes Suttirojpattana T., Somfai T., Matoba S., Parnpai R., Nagai T., Geshi M.	Animal Science Journal
Abstract: Our aim was to improve the developmental competence of bovine oocytes during their liquid storage by using additives. In vitro matured oocytes were stored for 20 h at 25°C in HEPES buffered TCM 199 medium (base medium). After storage, in vitro embryo development after in vitro fertilization was compared to those of non-stored (control) ones. Addition of 10% (v/v) newborn calf serum or 10.27 mmol/L pyruvate alone to the base medium did not improve blastocyst formation rates in stored oocytes; however, their simultaneous addition significantly improved the rate compared with those stored in base medium (P < 0.05). Supplementation of the holding medium with dithiothreitol (DTT) at any concentrations did not improve embryo development from stored oocytes. Although supplementation with cyclosporine A (CsA) significantly reduced apoptosis and membrane damage rates during storage, it did not improve the developmental competence of oocytes. 1,2-bis(2-aminophenoxy) ethane N,N,N’,N’-tetraacetic acid tetrakis-acetoxymethyl ester and ruthenium red had no effect on oocyte apoptotic rates. Blastocyst formation rates in all stored groups remained significantly lower than that of the control. In conclusion, pyruvate and serum had a synergic effect to moderate the reduction of oocyte quality during storage, whereas mitochondrial membrane pore inhibitor CsA and the antioxidant DTT did not affect their developmental competence. © 2016 The Authors. Animal Science Journal published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Animal Science Keyword: additives; bovine; in vitro fertilization; oocyte; storage Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84969785094&doi=10.1111%2fasj.12623&partnerID=40&md5=23e535cfdfbf9d99d48f754abea9bedd DOI: 10.1111/asj.12623
2017	Reversal of experimental liver damage after transplantation of stem-derived cells detected by FTIR spectroscopy Ye D., Heraud P., Parnpai R., Li T.	Stem Cells International
Abstract: The transplantation of autologous BM-MSCs holds great potential for treating end-stage liver diseases. The aim of this study was to compare the efficiency of transplanted rBM-MSCs and rBM-MSC-derived differentiated stem cells (rBM-MSC-DSCs) for suppression of dimethylnitrosamine-injured liver damage in rat model. Synchrotron radiation Fourier-transform infrared (SR-FTIR) microspectroscopy was applied to investigate changes in the macromolecular composition. Transplantation of rBM-MSC-DSCs into liver-injured rats restored their serum albumin level and significantly suppressed transaminase activity as well as the morphological manifestations of liver disease. The regenerative effects of rBM-MSC-DSCs were corroborated unequivocally by the phenotypic difference analysis between liver tissues revealed by infrared spectroscopy. Spectroscopic changes in the spectral region from 1190-970 cm-1 (bands with absorbance maxima at 1150 cm-1, 1081 cm-1, and 1026 cm-1) indicated decreased levels of carbohydrates, in rBM-MSC-DSC-transplanted livers, compared with untreated and rBM-MSC - transplanted animals. Principal component analysis (PCA) of spectra acquired from liver tissue could readily discriminate rBM-MSC-DSC-transplanted animals from the untreated and rBM-MSC-transplanted animals. We conclude that the transplantation of rBM-MSC-DSCs effectively treats liver disease in rats and SR-FTIR microspectroscopy provides important insights into the fundamental biochemical alterations induced by the stem-derived cell transplantation, including an objective "signature" of the regenerative effects of stem cell therapy upon liver injury. © 2017 Danna Ye et al. Keyword: Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042544690&doi=10.1155%2f2017%2f4585169&partnerID=40&md5=77e8e47dd8a164a94dae0af07fad49de DOI: 10.1155/2017/4585169
2017	Microtubule stabilisers docetaxel and paclitaxel reduce spindle damage and maintain the developmental competence of in vitro-mature bovine oocytes during vitrification Pitchayapipatkul J., Somfai T., Matoba S., Parnpai R., Nagai T., Geshi M., Vongpralub T.	Reproduction, Fertility and Development
Abstract: This study compared the efficacy of docetaxel (DT) and paclitaxel (PT) in reducing spindle damage during vitrification and maintaining the developmental competence of in vitro-matured (IVM) bovine oocytes after vitrification and warming. Pretreatment of IVM oocytes with 0.05M DT for 30min before vitrification resulted in significantly higher (P<0.05) rates of oocyte survival and cleavage after IVF, as well as subsequent blastocyst rates on Days 7-9 and hatching on Days 8-9, compared with oocytes pretreated with 1.0M PT before vitrification or those vitrified without pretreatment. When nuclear status and spindle morphology of vitrified oocytes were assess after warming by immunostaining, DT pretreatment before vitrification resulted in a significantly higher (P<0.05) percentage of oocytes at the MII stage with a normal, intact spindle compared with PT pretreatment or no pretreatment, but the percentage of MII oocytes was still significantly lower (P<0.05) than in the control group. Pretreatment of IVM bovine oocytes with 0.05M DT or 1.0M PT for 30min before vitrification reduces spindle damage to the same extent, without side effects on fertilisation and development. Pretreatment with 0.05M DT improved the developmental competence of vitrified-warmed oocytes to a greater degree than 1.0M PT pretreatment. © CSIRO 2017. Keyword: cattle; cryotolerance; cytoskeleton; disassembly; freezing; germplasm; metaphase II; taxanes Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029108414&doi=10.1071%2fRD16193&partnerID=40&md5=565133080236bf30effc6f2932b7f6b1 DOI: 10.1071/RD16193
2017	Enhanced chondrogenic differentiation of human umbilical cord wharton's jelly derived mesenchymal stem cells by GSK-3 Inhibitors Tanthaisong P., Imsoonthornruksa S., Ngernsoungnern A., Ngernsoungnern P., Ketudat-Cairns M., Parnpai R.	PLoS ONE
Abstract: Articular cartilage is an avascular, alymphatic, and aneural system with very low regeneration potential because of its limited capacity for self-repair. Mesenchymal stem cells (MSCs) are the preferred choice for cell-based therapies. Glycogen synthase kinase 3 (GSK-3) inhibitors are compounds that can induce the Wnt signaling pathway, which is involved in chondrogenesis and cartilage development. Here, we investigated the influence of lithium chloride (LiCl) and SB216763 synergistically with TGF-β3 on chondrogenic differentiation in human mesenchymal stem cells derived from Wharton's jelly tissue (hWJ-MSCs). hWJMSCs were cultured and chondrogenic differentiation was induced in monolayer and pellet experiments using chondrogenic medium, chondrogenic medium supplemented with LiCl, or SB216763 for 4 weeks. After in vitro differentiation, cultured cells were examined for the expression of Sox9, ACAN, Col2a1, and β-catenin markers. Glycosaminoglycan (GAG) accumulation was also examined by Alcian blue staining. The results indicated that SB216763 was more effective than LiCl as evidenced by a higher up-regulation of the expression of cartilage-specific markers, including Sox9, ACAN, Col2a1 as well as GAG accumulation. Moreover, collagen type II expression was strongly observed in cells cultured in the chondrogenic medium + SB216763 as evidenced by western blot analysis. Both treatments appeared to mediate the Wnt signaling pathway by up-regulating β-catenin gene expression. Further analyses showed that all treatments suppressed the progression of chondrocyte hypertrophy, determined by decreased expression of Col10a1 and Runx2. These results indicate that LiCl and SB216763 are potential candidates for further in vivo therapeutic trials and would be of great importance for cartilage regeneration. © 2017 Tanthaisong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Keyword: Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009104986&doi=10.1371%2fjournal.pone.0168059&partnerID=40&md5=f7ab44f2f5dce75bec27f77392f19599 DOI: 10.1371/journal.pone.0168059
2016	MIR-196a ameliorates cytotoxicity and cellular phenotype in transgenic huntington's disease monkey neural cells Kunkanjanawan T., Carter R.L., Prucha M.S., Yang J., Parnpai R., Chan A.W.S.	PLoS ONE
Abstract: Huntington's disease (HD) is an inherited neurodegenerative disorder caused by the expansion of polyglutamine (polyQ) tract that leads to motor, cognitive and psychiatric impairment. Currently there is no cure for HD. A transgenic HD nonhuman primate (HD-NHP) model was developed with progressive development of clinical and pathological features similar to human HD, which suggested the potential preclinical application of the HD-NHP model. Elevated expression of miR-196a was observed in both HD-NHP and human HD brains. Cytotoxicity and apoptosis were ameliorated by the overexpression of miR-196a in HD-NHP neural progenitor cells (HD-NPCs) and differentiated neural cells (HD-NCs). The expression of apoptosis related gene was also down regulated. Mitochondrial morphology and activity were improved as indicated by mitotracker staining and the upregulation of CBP and PGC-1α in HD-NPCs overexpressing miR-196a. Here we demonstrated the amelioration of HD cellular phenotypes in HD-NPCs and HD-NCs overexpressing miR-196a. Our results also suggested the regulatory role of miR-196a in HD pathogenesis that may hold the key for understanding molecular regulation in HD and developing novel therapeutics. © 2016 Kunkanjanawan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Keyword: Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84992385092&doi=10.1371%2fjournal.pone.0162788&partnerID=40&md5=d513d4fa551c9d66325c09eb34dfceba DOI: 10.1371/journal.pone.0162788
2016	Effect of hexavalent chromium-treated sperm on in vitro fertilization and embryo development Yoisungnern T., Das J., Choi Y.-J., Parnpai R., Kim J.-H.	Toxicology and Industrial Health
Abstract: Hexavalent chromium (Cr(VI)) is an environmental contaminant that is associated with reproductive abnormalities in both humans and animals. In the present study, we evaluated the cytotoxic effect of Cr(VI) on sperm function and subsequent embryo development after in vitro fertilization (IVF). Sperm obtained from BDF1 male mice were treated with potassium dichromate (0, 3.125, 6.25, 12.5, 25, or 50 μM) for 3 h. Cr(VI) significantly decreased sperm viability and acrosome reaction with increasing dose. These Cr(VI)-treated sperms were further used for IVF of oocytes obtained from BDF1 female mice. Results showed that Cr(VI)-treated sperm caused a significant reduction in IVF success, higher developmental arrest at the two-cell stage of embryos, and delayed blastocyst formation with increasing dose. In particular, most blastocysts from the Cr(VI)-treated sperm resulted in hatching failure as well as decreased inner cell mass and trophectoderm (TE). Furthermore, blastocysts obtained from Cr(VI)-treated sperm showed lower expression of not only TE-associated genes (eomes, cdx2, and krt8) but also pluripotent marker genes (sox2, pou5f1, and klf4) that are responsible for further embryo development of blastocyst embryos. The results of our current study showed that Cr(VI)-treated sperm had negative effects on oocyte fertilization and subsequent embryo development. © The Author(s) 2015. Keyword: acrosome reaction; Chromium; embryo development; gene expression; in vitro fertilization Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983604668&doi=10.1177%2f0748233715579805&partnerID=40&md5=890ddfdec6dccb0062770e9526d29e4e DOI: 10.1177/0748233715579805
2016	Vitrification of buffalo oocytes and embryos Parnpai R., Liang Y., Ketudat-Cairns M., Somfai T., Nagai T.	Theriogenology
Abstract: During the past decade, vitrification has been acknowledged as an efficient alternative to traditional slow-rate freezing in both human and animal embryology. The buffalo is the major milk and meat producing farm animal in many developing countries. Cryopreservation of buffalo oocytes and embryos is very important in preserving this species for future use. This review discusses the recent buffalo oocytes and embryos vitrification procedures, different types of cryoinjuries, and other factors affecting the vitrification of buffalo oocytes and embryos. © 2016 Elsevier Inc. Keyword: Buffalo; Embryos; Oocytes; Vitrification Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975268173&doi=10.1016%2fj.theriogenology.2016.04.034&partnerID=40&md5=3de1f95933eb93c4dfe5e3ebe6e43e96 DOI: 10.1016/j.theriogenology.2016.04.034
2016	The effect of temperature during liquid storage of in vitro-matured bovine oocytes on subsequent embryo development Suttirojpattana T., Somfai T., Matoba S., Nagai T., Parnpai R., Geshi M.	Theriogenology
Abstract: The aim of the present study was to optimize the temperature for the temporal storage of matured bovine oocytes. In vitro-matured bovine oocytes were preserved in HEPES-buffered TCM199 medium supplemented with 10% newborn calf serum at different temperatures (4 °C, 15 °C, 25 °C, and 38.5 °C) for 20 hours. Embryo development and blastocyst quality after in vitro fertilization, cytoplasmic ATP and glutathione levels in oocytes, and the frequency of apoptotic oocytes were compared among storage groups and a control group without storage. Among the storage groups, those at 25 °C and 38.5 °C showed the highest rates of blastocyst development (19.3% and 24.5%, respectively) compared with those stored at 4 °C and 15 °C (8.5% and 14.9%, respectively); however, blastocyst formation rates in all storage groups were lower than that in the control group (39.8%; P < 0.05). Storage at 38.5 °C and 15 °C was associated with reduced cell numbers in resultant blastocysts compared with the control and the 25 °C storage groups. Storage at 4 °C reduced metabolic activity of oocytes characterized by their lower ATP levels compared with the other groups. Storage for 20 hours significantly reduced the glutathione content in oocytes in all groups in a similar manner, irrespective of the temperature. Storage at 4 °C or 15 °C but not at 25 °C and 38.5 °C significantly increased the percentage of apoptotic oocytes compared with the control group. In conclusion, 25 °C was found to be the most suitable temperature for the temporal storage of matured bovine oocytes regarding both the developmental competence of oocytes and the quality of resultant blastocysts. © 2016 Elsevier Inc. Keyword: Aging; Bovine; Oocyte; Storage; Temperature Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84953299149&doi=10.1016%2fj.theriogenology.2015.09.033&partnerID=40&md5=f75f1c1f4d4d92bba5e2e0508bec8473 DOI: 10.1016/j.theriogenology.2015.09.033
2016	Pretreatment of bovine sperm with dithiobutylamine (DTBA) significantly improves embryo development after ICSI Suttirojpattana T., Somfai T., Matoba S., Nagai T., Parnpai R., Geshi M.	Journal of Reproduction and Development
Abstract: We assessed the effect of pretreating sperm with dithiobutylamine (DTBA) to improve embryo development by intracytoplasmic sperm injection (ICSI) in cows. Acridine Orange staining revealed that when applied at different concentrations (2.5, 5, and 10 mM) and exposure times (5 min, 20 min, 1 h, and 2 h), DTBA reduced disulfide bonds in spermatozoa with the highest efficacy at 5 mM for 5 min. DTBA enhanced the percentage of spermatozoa with free protamine thiol groups compared with untreated spermatozoa (control) (P < 0.05); however, this result did not differ from that of dithiothreitol (DTT) treatment. The percentage of live spermatozoa after DTBA treatment was identical to that in the control, but significantly higher than that after DTT treatment (P < 0.05). After ICSI, DTBA treatment tended to improve male pronuclear formation rate (P = 0.071) compared with non-treated sperm injection. Blastocyst formation rate was significantly improved by DTBA treatment compared with that in DTT, control, and sham injection groups (P < 0.05). Blastocyst quality in terms of cell numbers and ploidy was not different among these groups. In conclusion, DTBA increases the efficacy of blastocyst production by ICSI even if DTT treatment does not work. ©2016 by the Society for Reproduction and Development. Keyword: Bovine; Dithiobutylamine; Dithiothreitol; Intracytoplasmic sperm injection (ICSI) Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006728608&doi=10.1262%2fjrd.2016-084&partnerID=40&md5=1524316f636f7bbe02835112e35b79a5 DOI: 10.1262/jrd.2016-084