Associate Professor Rangsun Parnpai, Ph.D. Lecturer, School of Biotechnology
Contact: rangsun@g.sut.ac.th
Education
Dr.Agri.Sci. (Animal Reproduction), Kyoto University, Japan
M.Sc. (Zoology), Kasetsart University, Thailand
B.Sc. (Biology), Burapha University, Thailand
Area of Expertise
Somatic cell cloning in farm animals and endangered species
In vitro embryo production and embryo transfer in farm animals
Cryopreservation of gametes and embryos in farm animals
Human and animals stem cells biology and differentiation
Current Research
Somatic cell cloning in farm animals and endangered species
In vitro embryo production and embryo transfer in farm animals
Cryopreservation of gametes and embryos in farm animals
Human and animals stem cells biology and differentiation
Year
Month
Title
Journal
Information
2011
From microsurgery to single blastomere biopsy for ES cell establishment Lorthongpanich C., Laowtammathron C., Parnpai R.
Thai Journal of Veterinary Medicine
Abstract: Inner cell mass (ICM) is an important source of embryonic stem (ES) cells. There are several methods that have been developed to increase the efficiency of ICM cell isolation as well as improve the ES cells derivation rate. In conventional ICM isolation methods, the methods currently in use destroy trophectoderm cells of blastocyst stage embryos in order to release the ICM cell clumps. These conventional methods are considered embryo destruction methods as the embryos will be unable to survive after ICM is removed. Recently, an alternative method was reported using single biopsied blastomeres of earlier stage embryos as a source of ES cells. This novel method provides a new, ethically positive option that avoids destroying the embryos. This brief review of the techniques involved in ICM isolation and ES cells establishment, along with methodological comparisons, outlines the development of each technique, which may be used as a resource for choosing a suitable procedure for future experiments.
Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm Srirattana K., Matsukawa K., Akagi S., Tasai M., Tagami T., Nirasawa K., Nagai T., Kanai Y., Parnpai R., Takeda K.
Effects of Chemical Activation Treatment on Development of Swamp Buffalo (Bubalus bubalis) Oocytes Matured In Vitro and Fertilized by Intracytoplasmic Sperm Injection Liang Y.Y., Ye D.N., Laowtammathron C., Phermthai T., Nagai T., Somfai T., Parnpai R.
In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection Liang Y.Y., Phermthai T., Nagai T., Somfai T., Parnpai R.
Monkey hybrid stem cells develop cellular features of Huntington's disease Laowtammathron C., Cheng E.C.H., Cheng P.-H., Snyder B.R., Yang S.-H., Johnson Z., Lorthongpanich C., Kuo H.-C., Parnpai R., Chan A.W.S.
A comparison of cryotop and solid surface vitrification methods for the cryopreservation of In vitro matured bovine oocytes Sripunya N., Somfai T., Inaba Y., Nagai T., Imai K., Parnpai R.
Developmental rates of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection Liang Y., Phermthai T., Nagai T., Somfai T., Parnpai R.
Revista Veterinaria
Abstract: The objective of this study was to investigate the potential of swamp buffalo oocytes vitrified-warmed at the MII stage to develop to the blastocyst stage after parthenogenetic activation (PA) and intracytoplasmic sperm injection (ICSI). In the first experiment, we examined the toxic effects of cryoprotectants on in vitro development. In vitro matured oocytes were placed in 10% dimethylsulfoxide (DMSO) + 10% ethylene glycol (EG) for 1 min and then exposed to 20% DMSO + 20% EG + 0.5 M sucrose for 30 sec (1+30), 45 sec (1+45) or 60 sec (1+60). The oocytes were exposed to warming solution (0.5M sucrose) for 5 min and then washed in TCM199 HEPES + 20%FBS for 5 min. Oocyte viability was assessed by fluorescein diacetate (FDA) staining. Surviving oocytes were parthenogenetically activated and cultured for 7 days. The viability in all groups of CPA treatment and the control were 100%. The development rates to the blastocyst stage among CPA exposed 1+30 (17%), control (23%) and fresh control (control without FDA assay) (27%) did not differ significantly, but they were significantly higher than those in CPA exposed 1+45 (9%) and 1+60 (1%) groups. In the second experiment, we examined the effect of two CPA exposure times, 1+30 and 1+45 on the in vitro development for 7 days after PA of oocytes vitrified by the microdrop method. The viability in vitrified 1+30, 1+45 and the control groups was not different (97%, 95% and 100%, respectively). The development of surviving oocytes to blastocyst stage in the vitrified 1+30 group (8%) was significantly higher than that in the vitrified 1+45 group (4%) and significantly lower than those in control and fresh control groups (24% and 26%, respectively). In the third experiment, we examined the effect of two CPA exposure times, 1+30 and 1+45 on in vitro development after ICSI of vitrified oocytes. The FDA viability in vitrified 1+30, 1+45 and control groups (100%) was not different (96%, 91% and 100%, respectively). After ICSI vitrified-warmed oocytes were activated and oocytes with the 2nd polar body were cultured for 7 days. The development of ICSI oocytes to the blastocyst stage in the vitrified 1+30 group (11%) was significantly higher than that in vitrified 1+45 (7%) and significantly lower than those in the control and fresh control (21% and 23%, respectively). In conclusion, our study demonstrated that the 1+30 CPA treatment regimen could yield the highest blastocyst rates for oocytes vitrified by the microdrop method and that the FDA viability test had no effect on the embryo development.
Allelic switching of the imprinted IGF2R gene in cloned bovine fetuses and calves Suteevun-Phermthai T., Curchoe C.L., Evans A.C., Boland E., Rizos D., Fair T., Duffy P., Sung L.Y., Du F., Chaubal S., Xu J., Wechayant T., Yang X., Lonergan P., Parnpai R., Tian X.C.
FTIR microspectroscopic imaging as a new tool to distinguish chemical composition of mouse blastocyst Thumanu K., Tanthanuch W., Lorthongpanich C., Heraud P., Parnpai R.
