Year
Month
Title
Journal
Information
2008
Chemical enhancement in embryo development and stem cell derivation from single blastomeres
Lorthongpanich C., Yang S.-H., Piotrowska-Nitsche K., Parnpai R., Chan A.W.S.
Cloning and Stem Cells
View
Abstract:
Several chemicals targeting the mitogen-activated protein (MAP) kinase signaling pathway, which play an important role in regulating cell growth and differentiation, have shown enhancing effects on the development of the inner cell mass (ICM) and the derivation of ES cells. However, investigation of such chemicals on early embryonic development and the establishment of ES cell lines has not been elucidated. This study was aimed to determine if ACTH, MAP2K1 inhibitor [MAP2K1 (I)], and MAPK14 inhibitor [MAPK14 (I)] could enhance the development of the ICM in preimplantation mouse embryos and blastocyst outgrowths, and the establishment of ES cell lines from blastomeres of early embryos. We have demonstrated that both MAP2K1 (I) and MAPK14 (I) delay early embryo development and inhibit the development of embryos from early blastomeres. On the other hand, ACTH had a positive effect on embryos derived from early blastomeres. As a result, 17 ES cell lines were established. Among these ES cell lines, nine and five ES cell lines were established from single blastomeres of two-cell embryos with and without the supplement of ACTH, respectively. In addition to two-cell isolated blastomeres, three ES cell lines were established from blastomeres of four-cell embryos only with the supplement of ACTH. Our results suggest that ACTH can enhance the derivation of ES cells from single blastomere-derived embryos. © Mary Ann Liebert, Inc. 2008.
Keyword:
Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-57149109568&doi=10.1089%2fclo.2008.0035&partnerID=40&md5=91443cf282ebc5641168352de0e02ad7
DOI: 10.1089/clo.2008.0035
2008
Erratum: Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells (Reproduction vol. 135 (6) (808))
Lorthongpanich C., Yang S.-H., Piotrowska-Nitsche K., Parnpai R., Chan A.W.S.
Reproduction
View
2008
Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells
Lorthongpanich C., Yang S.-H., Piotrowska-Nitsche K., Parnpai R., Chan A.W.S.
Reproduction
View
Abstract:
The recently developed technique of establishing embryonic stem (ES) cell lines from single blastomeres (BTMs) of early mouse and human embryos has created significant interest in this source of ES cells. However, sister BTMs of an early embryo might not have equal competence for the development of different lineages or the derivation of ES cells. Therefore, single BTMs from two- and four-cell embryos of outbred mice were individually placed in sequential cultures to enhance the formation of the inner cell mass (ICM) and the establishment of embryonic outgrowth. The outgrowths were then used for the derivation of ES cell lines. Based on the expression of ICM (Sox2) and trophectoderm (Cdx2) markers, it was determined that ICM marker was lacking in blastocysts derived from 12% of BTMs from two-cell stage and 20% from four-cell stage. Four ES cell lines (5.6%; 4/72) were established ater culture of single BTMs from two-cell embryos, and their pluripotency was demonstrated by their differentiation into neuronal cell types. Our results demonstrate that sister BTMs of an early embryo are not equally competent for ICM marker expression. However, we demonstrated the feasibility of establishing ES cells from a single BTM of outbred mice. © 2008 Society for Reproduction and Fertility.
Keyword:
Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-45249121799&doi=10.1530%2fREP-07-0478&partnerID=40&md5=49bd8ad5408a3ff919256d979bc7368f
DOI: 10.1530/REP-07-0478
2007
Production of cloned embryos in buffalo
Parnpai R.
Italian Journal of Animal Science
View
2006
Epigenetic characteristics of cloned and in vitro-fertilized swamp buffalo (Bubalus bubalis) embryos
Suteevun T., Parnpai R., Smith S.L., Chang C.-C., Muenthaisong S., Tian X.C.
Journal of Animal Science
View
Abstract:
Swamp buffalos are becoming endangered due to reproductive inefficiencies. This is of concern because many countries depend heavily on their products. Somatic cell nuclear transfer (SCNT) is a potential strategy for preserving endangered species. To date, SCNT in swamp buffalo has succeeded in the creation of blastocyst embryos. However, development to term of SCNT swamp buffalos is extremely limited, and only 1 live birth has been reported. An abnormal epigenetic mechanism is suspected to be the cause of developmental failure, as is also seen in other species. The DNA methylation and histone acetylation are key players in epigenetic modification and display marked variability during embryonic preimplantation development. Knowledge of epigenetic modifications will aid in solving the developmental problems of SCNT embryos and improving reproductive technology in the swamp buffalo. The objective of this study was to determine the relationship between preimplantation embryonic development and 2 epigenetic patterns, global DNA methylation and histone acetylation, in SCNT and in vitro-fertilized (IVF) swamp buffalo embryos. In addition, we examined the correlations between those 2 mechanisms in the SCNT and IVF swamp buffalo embryos throughout the developmental stages using double immunostaining and quantification of the emission intensities using confocal microscopy. We discovered an aberrant methylation pattern in early preimplantation-stage swamp buffalo SCNT embryos. In addition, greater variability in the DNA methylation levels among nuclei within SCNT embryos was discovered. Hyperacetylation was also observed in SCNT embryos compared with IVF embryos at the 4- and 8-cell stages (P < 0.05). Dynamic changes and interplay between these 2 epigenetic mechanisms could be crucial for embryonic development during the early preimplantation period. The aberrancies uncovered here may contribute to the low efficiency of SCNT. © 2006 American Society of Animal Science. All rights reserved.
Keyword: DNA methylation; Epigenetics; Histone acetylation; Nuclear transfer; Preimplantation embryo; Swamp buffalo
Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748422110&doi=10.2527%2fjas.2005-695&partnerID=40&md5=8ddb2f9f12f3658beacce8e06622fc30
DOI: 10.2527/jas.2005-695
2006
Anomalous mRNA levels of chromatin remodeling genes in swamp buffalo (Bubalus bubalis) cloned embryos
Suteevun T., Smith S.L., Muenthaisong S., Yang X., Parnpai R., Tian X.C.
Theriogenology
View
Abstract:
The swamp buffalo (Bubalus bubalis) is a multi-purpose animal in agriculture that is challenged by extinction due to low reproductive efficiency. Nuclear transfer (NT) has been used to preserve special breeds of buffalo, as well as to increase the number of animals. However, cloned buffalo embryos have impaired development, as in other species. To understand the chromatin remodeling activities in cloned embryos and to improve NT technology, we examined the expression profiles of five genes involved in DNA and histone modifications, DNMT1, DNMT3A, DNMT3B, HAT1 and HDAC1, in single swamp buffalo metaphase II oocytes, NT and in vitro fertilized (IVF) embryos from the two-cell to the blastocyst stage, by quantitative real time RT-PCR. We observed similar expression dynamics for all genes studied in the NT and IVF embryos: relatively constant levels of expression for all genes were found from the MII oocyte up to the eight-cell stage; the levels of mRNA for HAT1 and DNMT3B continued to be stably expressed up to the blastocyst stage; while dramatic increases were seen for DNMT3A and HDAC1. Alternatively, the levels of DNMT1 started to decrease at the eight-cell stage. Despite the similarity in the dynamics of gene expression, dramatic differences in the relative levels of these genes between NT and IVF embryos were observed. The expression levels of all DNA modifying genes were higher in the NT embryos than in the IVF embryos at the eight-cell and blastocyst stages. The genes HDAC1 and HAT1 were also expressed significantly higher at the blastocyst stage in the NT embryos. Our results suggested differences in chromatin remodeling between NT and IVF embryos and that lower levels of DNA passive demethylation and higher levels of DNA de novo methylation occurred in the NT embryos. These observations are novel in the species of buffalo, and may be associated with developmental failure of cloned buffalo embryos due to the transcriptional repression effect of most genes studied here. © 2005 Elsevier Inc. All rights reserved.
Keyword: DNMT; Embryo development; Epigenetic; Histone acetylases; Preimplantation
Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-33646187381&doi=10.1016%2fj.theriogenology.2005.09.015&partnerID=40&md5=98c5b65a98b6e59a36ef62c148552b8d
DOI: 10.1016/j.theriogenology.2005.09.015
1998
Effets of Leukemia Inhibitory Factor, Stem Cell Factor, and Basic Fibroblast Growth Factor on the Proliferation of Bovine ICM Cells in Vitro
Parnpai R., Fujikawa M., Minami N., Yamada M., Utsumi K.
Journal of Mammalian Ova Research
View
Abstract:
Experiments were conducted to determine the effects of leukemia inhibitory factor (LIF), stem cell factor (SF), and basic fibroblast growth factor (bFGF) on the proliferation of undifferentiated primitive ectodermal cells isolated from bovine inner cell masses (ICM). ICMs of bovine hatched blastocysts produced at 10 days post-insemination (dpi) were immunosurgically isolated. Either one or three ICMs were cultured on mouse embryonic fibroblasts (MEF) in a 100 μl droplet of control medium with or without several combinations of murine recombinant LIF (1,000 units/ml), human recombinant SF (40 ng/ml), and human recombinant bFGF (40 ng/ml) as follows: 1) LIF, 2) SF, 3) bFGF, 4) LIF+SF, 5) LIF+bFGF, 6) SF+bFGF and 7) LIF+SF+bFGF at 37°C under 5% CO2 in air. Attachment of ICMs to a feeder layer and proliferation of ectodermal cells were observed daily. After 6 to 7 days of culture, the primary ectodermal cells outgrown from ICM clumps were mechanically isolated with a 26G needle and mechanically disaggregated by pipetting through a fine pipette to make small clumps of cells. The primary ectodermal cell clumps were cultured and serially passaged every 6 to 7 days. The attachment of isolated ICMs started 1 day after culture in each treatment, and the rates of attachment ranged from 74 to 94%. The rates of ectodermal cells outgrowing from 1 ICM and 3 ICMs (NULL,37 and 56% respectively) were higher in the LIF+SF containing medium than those in the control medium and bFGF containing medium (16 and 17%; 22 and 22% respectively). When primary ectodermal cells that had been isolated from ICMs were cultured in the LIF+SF containing medium, the undifferentiated ectodermal cells were serially passaged (6 passages) during 2 months in culture. The results of this study demonstrate that 1) groups of 3 ICMs gave superior results to single ICM for the formation of ectodermal cell colonies from the ICM clumps and 2) LIF and SF are important factors in supporting the primary outgrowth and serial passage of ectodermal cells, as well as for maintaining an undifferentiated state as determined by a histochemical staining of alkaline phosphatase. © 1998, JAPANESE SOCIETY OF OVA RESEARCH. All rights reserved.
Keyword: bFGF; Bovine ICM cells; LIF; Proliferation in vitro; SF
Scopus Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85024450773&doi=10.1274%2fjmor.15.132&partnerID=40&md5=7e02ff54eb71f85e41dac1aa2943a615
DOI: 10.1274/jmor.15.132
1983
Plasma progesterone, oestrone and oestrone sulphate levels during the first half of gestation in swamp buffaloes.
Kamonpatana M., Parnpai R., Ngramsuriyaroj C., Srisakwattana K.
The British veterinary journal
View
